This study aim ed to explore the penetration of some mid Asian sheep blood s into Chinese indigenous sheep breeds. Applying the method of random sampling in the typical colonies of the central area of habitat, the genetic variation s of 5 structural gene loci (Tf, Ary Es, Hb ?, Xp, Ke) in 10 representative Chinese sheep breeds , Sishui Fur, Sunite, Wurank, Bayinbuluk , Altay , Small Tailed Han, Wadi, Tan, Tong and Hu sheep were examined using various electrophoric methods and Na/K/Cl ion detector . The data were then compared with those of other 1 2 Asian sheep populations examined by the same experimental method s . The phylogenetic relationship of these 2 2 popul ations were then analy z ed based on the genetic distance and cluster analysis. The average heterozygosity (H) of Sheep populations in this study was 0.3954, the average polymorphic information content(PIC) valued 0.34 , t he average number of effective allele (Ne) were 1.9624 , indicating a relatively high genetic differentiation at the 5 structural loci in the 10 sheep populations Phylogentic analysis exposed that all the 10 sheep populations belonged to the Mongolian sheep lineage. However, t he occurrence of certain alleles , originally the advantageous alleles of Tibetan sheep lineage or South Southeast Asian sheep lineage, in the 10 populations , like HbA and TfF, wa s evidence for the penetration of mid Asian blood into Chinese indigenous sheep populations. Al l the 10 Chinese sheep breeds belonged to "Mongolian sheep" lineage , but with some pen e tration of Tibetan sheep lineage and/or South Southeast Asian sheep lineage.